Based on molecular identification, the fungus Aspergillus sydowii and the bacterium Nevskia terrae, with accession numbers MG991624 and AB806800, respectively, were isolated and identified from an antiquated manuscript formed from cellulosic fibers. The highest values of mechanical characteristics were determined to be in CMC/PGP/TiO 2-1 with values of 25.4 g, 6.6 MPa, and 11.4%, for tensile strength, elongation at break, and tear strength, respectively. The results showed that the unique deposition of TiO 2NPs on the biofilm surface gave the produced films loaded with TiO 2NPs a rough structure. ![]() The synthesized TiO 2NPs were nearly spherical in shape, small in size (98 nm), and stable (zeta potential value − 33 mV). Three volumes (1, 2, 4 mL) of TiO 2NPs were put into a biofilm combination to produce the poultices known as CMC/PGP/TiO 2-1, CMC/PGP/TiO 2-2, and CMC/PGP/TiO 2-3. ![]() As a result, a simple method was created that uses poultice biofilm made of carboxymethyl cellulose (CMC) and Phytagel plant cell (PGP) loaded with TiO 2NPs to preserve manuscripts against microbes in an environmentally responsible way. In this study, TiO 2 nanoparticle (TiO 2NP)-coated film was produced to protect manuscripts against microorganisms using ecofriendly benign materials.
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